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The effect of finasteride on spermatogenesis of Mesocricetus auratus
Dimas José Araújo VidigalI, Alcino Lázaro da SilvaII, Luiz Mauro Andrade da FonsecaIII, Anilton Cesar VasconcelosIV,
Dilermando Fazito de ResendeVI, Felipe Eduardo Costa VidigalVI.
I Fellow PhD degree in Surgery, Medicine School, Federal University of Minas Gerais (UFMG), Brazil.
II Emeritus Professor, UFMG, Brazil.
III Full Professor, Department of Pathology, Medicine School of Barbacena, “José Bonifácio” Foundation (FAME-FUNJOB), Minas Gerais, Brazil.
IV Associate Professor, Laboratory of Apoptosis, Department of General Pathology, Institute of Biological Science, UFMG, Brazil.
V Full Professor, Scientific Methodology, FAME-FUNJOB, Minas Gerais, Brazil.
VI Graduate Student, SUPREMA, School of Medicine, Juiz de Fora, Minas Gerais, Brazil.
1. Research performed at Post-Graduation Program in Surgery, Medicine School, Federal University of Minas Gerais (UFMG) and Laboratory of Apoptosis,
Department of General Pathology, Institute of Biological Science, UFMG, Institute of Bio-Science, Presidente “Antônio Carlos” University (INBIO-UNIPAC).
ABSTRACT
Purpose: To study the effect of finasteride on the spermatogenesis of adult Mesocricetus auratus.
Methods: Twenty adult hamsters were evaluated. The animals were one year-older, and were randomly divided in 2 different groups: control group with ten animals (n=10) and experimental group also with ten animals (n=10). The animals in the experimental group were shot 7.14 ng/mL (0.5mL) of finasteride by 100mg/Kg, subcutaneously in the dorsal region three times per week during 90 days. This dose correspondes to 5mg of the drug used in adult men for the treatment of benign prostatic hyperplasia (BPH). After three months, the animals were anesthetized through association of 200mg/kg ketamine chloridrate and 2.5 mg/kg of diazepan and were dead through hypovolemia.. The testis removed along with the whole genitourinary apparel were fixed with 10% formalin and submitted to histological analisys by optical microscopy. The hematoxilin-eosin (HE) method was used to stain the slides.
Results: The mean weight of animals in the control group before death was 129.0±18.8gr. The mean weight of animals in experimental group was 145.0±15.25gr. The mean age of animals in control group before death was 15.2±1.13 months. The mean age of animals in experimental group before death was 17.16±0.82 months. The mean difference in weight between both groups was not statistical significant (p=0.0514). The totality of animals in control group (100%) presented no tubular alterations and showed no disturbancy
in the spermatogenesis stages. Four animals (40%) in the experimental group showed hypotrophy of the seminiferous tubules and six (60%) showed normal spermatogenesis, however reduced compared to control group. There was statiscally significant difference (p=0.043) between the control and experimental group related to testicular alterations.
Conclusion: The animals that were administered finasteride showed significant tubules atrophy and spermatogenesis reduction compared to control group.
Dimas José Araújo VidigalI, Alcino Lázaro da SilvaII, Luiz Mauro Andrade da FonsecaIII, Anilton Cesar VasconcelosIV,
Dilermando Fazito de ResendeVI, Felipe Eduardo Costa VidigalVI.
I Fellow PhD degree in Surgery, Medicine School, Federal University of Minas Gerais (UFMG), Brazil.
II Emeritus Professor, UFMG, Brazil.
III Full Professor, Department of Pathology, Medicine School of Barbacena, “José Bonifácio” Foundation (FAME-FUNJOB), Minas Gerais, Brazil.
IV Associate Professor, Laboratory of Apoptosis, Department of General Pathology, Institute of Biological Science, UFMG, Brazil.
V Full Professor, Scientific Methodology, FAME-FUNJOB, Minas Gerais, Brazil.
VI Graduate Student, SUPREMA, School of Medicine, Juiz de Fora, Minas Gerais, Brazil.
1. Research performed at Post-Graduation Program in Surgery, Medicine School, Federal University of Minas Gerais (UFMG) and Laboratory of Apoptosis,
Department of General Pathology, Institute of Biological Science, UFMG, Institute of Bio-Science, Presidente “Antônio Carlos” University (INBIO-UNIPAC).
ABSTRACT
Purpose: To study the effect of finasteride on the spermatogenesis of adult Mesocricetus auratus.
Methods: Twenty adult hamsters were evaluated. The animals were one year-older, and were randomly divided in 2 different groups: control group with ten animals (n=10) and experimental group also with ten animals (n=10). The animals in the experimental group were shot 7.14 ng/mL (0.5mL) of finasteride by 100mg/Kg, subcutaneously in the dorsal region three times per week during 90 days. This dose correspondes to 5mg of the drug used in adult men for the treatment of benign prostatic hyperplasia (BPH). After three months, the animals were anesthetized through association of 200mg/kg ketamine chloridrate and 2.5 mg/kg of diazepan and were dead through hypovolemia.. The testis removed along with the whole genitourinary apparel were fixed with 10% formalin and submitted to histological analisys by optical microscopy. The hematoxilin-eosin (HE) method was used to stain the slides.
Results: The mean weight of animals in the control group before death was 129.0±18.8gr. The mean weight of animals in experimental group was 145.0±15.25gr. The mean age of animals in control group before death was 15.2±1.13 months. The mean age of animals in experimental group before death was 17.16±0.82 months. The mean difference in weight between both groups was not statistical significant (p=0.0514). The totality of animals in control group (100%) presented no tubular alterations and showed no disturbancy
in the spermatogenesis stages. Four animals (40%) in the experimental group showed hypotrophy of the seminiferous tubules and six (60%) showed normal spermatogenesis, however reduced compared to control group. There was statiscally significant difference (p=0.043) between the control and experimental group related to testicular alterations.
Conclusion: The animals that were administered finasteride showed significant tubules atrophy and spermatogenesis reduction compared to control group.
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