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Titre du document / Document title
Curcumin down-regulates AR gene expression and activation in prostate cancer cell lines
Auteur(s) / Author(s)
NAKAMURA Keiichiro (1) ; YASUNAGA Yutaka (1) ; SEGAWA Takehiko (1) ; DAEJIN KO (1) ; MOUL Judd W. (1) ; SRIVASTAVA Shiv (1) ; RHIM Johng S. (1) ;
Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)
(1) Center for Prostate Disease Research, Department of Surgery, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, ETATS-UNIS
Résumé / Abstract
Curcumin, traditionally used as a seasoning spice in Indian cuisine, has been reported to decrease the proliferation potential of prostate cancer cells, by a mechanism that is not fully understood. In the current study, we have evaluated the effects of curcumin in cell growth, activation of signal transduction, and transforming activities of both androgen-dependent and independent cell lines. Prostate cancer cell lines, LNCaP and PC-3, were treated with curcumin and its effects were further analyzed on signal transduction and expression of androgen receptor (AR) and AR-related cofactors using transient transfection assay and Western blotting. Our results show that curcumin down-regulates transactivation and expression of AR, activator protein-1 (AP-1), nuclear factor-KB (NF-KB), and CREB (cAMP response element-binding protein)-binding protein (CBP). Curcumin also inhibited the transforming activities of both cell lines as evidenced by the reduced colony forming ability in soft agar. The results obtained here demonstrate that curcumin has a potential therapeutic effect on prostate cancer cells through down-regulation of AR and AR-related cofactors (AP-1, NF-KB and CBP).
Revue / Journal Title
International journal of oncology ISSN 1019-6439
Source / Source
2002, vol. 21, no4, pp. 825-830
Curcumin down-regulates AR gene expression and activation in prostate cancer cell lines
Auteur(s) / Author(s)
NAKAMURA Keiichiro (1) ; YASUNAGA Yutaka (1) ; SEGAWA Takehiko (1) ; DAEJIN KO (1) ; MOUL Judd W. (1) ; SRIVASTAVA Shiv (1) ; RHIM Johng S. (1) ;
Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)
(1) Center for Prostate Disease Research, Department of Surgery, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, ETATS-UNIS
Résumé / Abstract
Curcumin, traditionally used as a seasoning spice in Indian cuisine, has been reported to decrease the proliferation potential of prostate cancer cells, by a mechanism that is not fully understood. In the current study, we have evaluated the effects of curcumin in cell growth, activation of signal transduction, and transforming activities of both androgen-dependent and independent cell lines. Prostate cancer cell lines, LNCaP and PC-3, were treated with curcumin and its effects were further analyzed on signal transduction and expression of androgen receptor (AR) and AR-related cofactors using transient transfection assay and Western blotting. Our results show that curcumin down-regulates transactivation and expression of AR, activator protein-1 (AP-1), nuclear factor-KB (NF-KB), and CREB (cAMP response element-binding protein)-binding protein (CBP). Curcumin also inhibited the transforming activities of both cell lines as evidenced by the reduced colony forming ability in soft agar. The results obtained here demonstrate that curcumin has a potential therapeutic effect on prostate cancer cells through down-regulation of AR and AR-related cofactors (AP-1, NF-KB and CBP).
Revue / Journal Title
International journal of oncology ISSN 1019-6439
Source / Source
2002, vol. 21, no4, pp. 825-830